Using familial information for variant filtering in high-throughput sequencing studies
نویسندگان
چکیده
منابع مشابه
Data-based filtering for replicated high-throughput transcriptome sequencing experiments
MOTIVATION RNA sequencing is now widely performed to study differential expression among experimental conditions. As tests are performed on a large number of genes, stringent false-discovery rate control is required at the expense of detection power. Ad hoc filtering techniques are regularly used to moderate this correction by removing genes with low signal, with little attention paid to their ...
متن کاملAccurate multiplexing and filtering for high-throughput amplicon-sequencing
Tagging amplicons with tag sequences appended to PCR primers allow the multiplexing of numerous samples for high-throughput sequencing (HTS). This approach is routinely used in HTS-based diversity analyses, especially in microbial ecology and biomedical diagnostics. However, amplicon library preparation is subject to pervasive sample sequence cross-contaminations as a result of tag switching ev...
متن کاملStudies on Human Chromatin using High-Throughput DNaseI Sequencing
Bioinformatics) Studies on Human Chromatin using High-Throughput DNaseI Sequencing
متن کاملA novel conceptual approach to read-filtering in high-throughput amplicon sequencing studies
Adequate read filtering is critical when processing high-throughput data in marker-gene-based studies. Sequencing errors can cause the mis-clustering of otherwise similar reads, artificially increasing the number of retrieved Operational Taxonomic Units (OTUs) and therefore leading to the overestimation of microbial diversity. Sequencing errors will also result in OTUs that are not accurate rec...
متن کاملEstimating Protistan Diversity Using High-Throughput Sequencing.
Sequencing hypervariable regions from the 18S rRNA gene is commonly employed to characterize protistan biodiversity, yet there are concerns that short reads do not provide the same taxonomic resolution as full-length sequences. A total of 7,432 full-length sequences were used to perform an in silico analysis of how sequences of various lengths and target regions impact downstream ecological int...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Human Genetics
سال: 2014
ISSN: 0340-6717,1432-1203
DOI: 10.1007/s00439-014-1479-4